Immobilized enzyme

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An immobilized enzyme is an enzyme that is attached to an inert, insoluble material. This can provide a higher resistance to changes in condition such as pH or temperature.

Commercial use[change | change source]

Immobilised enzymes can be important for commercial uses as they have many benefits to the cost and processes of the reaction such as:

  • Convenience: small amounts of protein dissolve in the reaction so purification of the product is easier.
  • Economy: The immobilized enzyme is easily removed from the reaction so it can be recycled. This is useful in the production of lactose-free milk as the milk can be drained from a container leaving the enzyme (lactase) inside for the next batch.
  • Stability: Immobilised enzymes normally have a higher thermal and operational stability than the soluble form of the enzyme.[1]
  • Safety: In the past, biological washing powders contained proteases and lipases that would break down dirt but also irritate the skin if it was touched - immobilization prevents this from happening

Methods[change | change source]

  • Adsorption on glass: The enzyme is attached to a glass bead.
  • Covalent bond: The enzyme is bound covalently to a support (such as silica gel).
  • Cross-linkage: Multiple enzymes are bound together to create a matrix.
  • Affinity-tag binding: Enzymes attached to a surface with protein tags.

References[change | change source]

  1. Wu, Hong; Liang, Yanpeng; Shi, Jiafu; Wang, Xiaoli; Yang, Dong; Jiang, Zhongyi (April 2013). "Enhanced stability of catalase covalently immobilized on functionalized titania submicrospheres". Materials Science and Engineering: C. 33 (3): 1438–1445. doi:10.1016/j.msec.2012.12.048.