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This article lists the fifty most-subscribed channels on the video platform YouTube. The ability to subscribe to users was introduced in October 2005, and the website began publishing a list of its "most subscribed Members" in April 2006. An early archive of the list dates to May 2006, at which time Smosh, with fewer than three thousand subscribers, occupied the number-one position. The most-subscribed channel as of August 2023 is T-Series, which has accumulated more than 247 million subscribers since 2006.
More than ten different channels have been the most-subscribed in the history of YouTube: Mr. Beast, Smosh, Judson Laipply, Brookers, geriatric1927, lonelygirl15, nigahiga, Fred, Ray (formerly RayWilliamJohnson), PewDiePie, YouTube (formerly YouTube Spotlight), and T-Series.