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Substrate may mean:
- Substrate (biology), the natural environment in which an organism lives, or the surface or medium on which an organism grows or is attached
- Substrate (marine biology), the earthy material in the bottom of a marine habitat, like dirt, rocks, sand, or gravel
- Substrate (aquarium), the material used in the bottom of an aquarium
- The stratum that is covered by another geologic stratum
- Substrate (biochemistry), a molecule an enzyme has an effect on
- Substrate (chemistry), the catalytic material upon which chemical species react
Substrate may also mean:
- Wafer (electronics), material upon which integrated circuits are fabricated
- Printed circuit board (electronics), or the electrically insulating portion of a PCB structure
- Substratum, in linguistics, a language that influences but is supplanted by a second language
- Neural substrate, in neuroscience, the set of brain structures that underlies a specific behavior or psychological state
- Substrate (printing), the base material to which images will be printed
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