Immunohistochemistry

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Immunohistochemistry (IHC) it involves the process of selectively identifying antigens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.[1][2] IHC takes its name from the roots "immuno", which simply means antibodies used in the procedure, and "histo", means. Immunohistochemistry was been conceptualized by Albert Coons.[3][4]

Chromogenic immunohistochemistry of a normal kidney targeting the protein CD10.

Sample Preparation[change | change source]

This involve proper tissue collection, fixation and sectioning. A solution of formalin is often used to fix tissue, but other methods may also be used.

References[change | change source]

  1. https://doi.org/10.1177%2F0300985813505879
  2. "Overview of Immunohistochemistry - US". www.thermofisher.com. Retrieved 2021-11-21.
  3. "immunohistochemistry". www.cancer.gov. 2011-02-02. Retrieved 2021-11-21.
  4. "Immunohistochemistry Principle & Protocol". Bosterbio. Retrieved 2021-11-21.