Middelburg may refer to:
- Middelburg, Belgium, a neighborhood of Maldegem, Province of East Flanders, Belgium
- Middelburg, Eastern Cape, a town in the Province of Eastern Cape, South Africa
- Middelburg, Mpumalanga, a city in the Province of Mpumalanga, South Africa
- Middelburg, North Holland, a hamlet in the Province of South Holland, the Netherlands
- Middelburg, Zeeland, capital of the Province of Zeeland, the Netherlands
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