Because cell walls are made differently in the different kingdoms, different enzymes are used to remove them.
Enzymes for the preparation of protoplasts[change | change source]
|Type of cell||Enzyme|
|Plant cells||Cellulase, pectinase, xylanase|
|Gram-positive bacteria||Lysozyme (+EDTA)|
The reason for removing the cell walls is to allow many different experimental techniques to be used.
Protoplasts are widely used for DNA transformation (for making genetically modified organisms), since the cell wall would otherwise block the passage of DNA into the cell. In the case of plant cells, protoplasts can be regenerated into whole plants.
References[change | change source]
- Davey M.R. et al 2005. "Plant protoplasts: status and biotechnological perspectives". Biotechnol. Adv. 23 (2): 131–71. doi:10.1016/j.biotechadv.2004.09.008. PMID 15694124.CS1 maint: uses authors parameter (link)
- Thorpe T.A. 2007. "History of plant tissue culture". Mol. Biotechnol. 37 (2): 169–80. doi:10.1007/s12033-007-0031-3. PMID 17914178.