The Meselson–Stahl experiment was an experiment done by Matthew Meselson and Franklin Stahl in 1958, using E. coli DNA. With their experiment, they concluded that DNA replication was semiconservative. The DNA consists of two helices that are combined. When the two helices are copied, each will have one part coming from the original cell. The other part will be newly made. In chemistry, this is called synthesis.
There are three different way this replication could happen:
- conservative replication: The original DNA is kept as it is. Two new helices will be synthesized from it.
- semi-conservative replication: Half of the DNA is kept, the other half is synthesized.
- disperse replication: The mechanism is the same as semi-conservative replication; but the synthesized links could be added to either the old or new helix.
Meselson and Stahl concluded that DNA employs semi-conservative replication. They placed the bacteria in an environment which contained a selected Nitrogen isotope. They first used N14. The bacteria then integrated this isotope into their DNA. Later they used an environment that contained N15. They then looked at which of the isotopes the bacterial DNA contained. They found that it contained both isotopes of nitrogen, which implies that conservative replication is not the correct conclusion. By having a closer look they could then also rule out disperse replication. Some helix strands were all N14. Other helix strands from the time in the N15 environment had N15.
References[change | change source]
- Meselson, M. and Stahl, F.W. (1958). "The Replication of DNA in Escherichia coli". PNAS 44: 671–82.