Directed evolution (DE) is a method used to produce enzymes for industrial or medical purposes.
Testing more mutants increases the chances of finding one with the desired properties.
During in vivo evolution, each cell (usually bacteria or yeast) is transformed with a plasmid containing a different member of the variant library. Only the gene of interest differs between the cells, with all other genes being kept the same.
The cells express the protein either in their cytoplasm or surface where its function can be tested. This format has the advantage of selecting for properties in a cellular environment, which is useful when the evolved protein or RNA is to be used in living organisms.
When done without cells, DE uses in vitro transcription translation to produce proteins or RNA free in solution or inside artificial microdroplets. This has the benefit of allowing more conditions (e.g. temperature, solvents). It can express proteins that would be toxic to cells. Furthermore, in vitro evolution experiments can generate far larger libraries (up to 1015) because the library DNA need not be inserted into cells. That often limits what can be done.
Ensuring heredity[change | change source]
Alternatively the protein and its gene can be kept together, or in emulsion droplets. The gene sequences isolated are then multiplied by PCR or by transformed host bacteria. Either the single best sequence, or a pool of sequences can be used as the template for the next round of mutagenesis. The repeated cycles of diversification-selection-amplification make enzyme variations adapted to the selection process.
Prize awarded[change | change source]
References[change | change source]
- Stephen Lutz 2010. Beyond directed evolution - semi-rational protein engineering and design. Curr Opin Biotechnol. 21(6): 734–743.
- Voigt C.A; Kauffman S. & Wang Z.G. 2000. "Rational evolutionary design: the theory of in vitro protein evolution". Advances in protein chemistry. 55: 79–160. doi:10.1016/s0065-3233(01)55003-2. PMID 11050933.CS1 maint: multiple names: authors list (link)
- Dalby P.A. 2011. "Strategy and success for the directed evolution of enzymes". Current Opinion in Structural Biology. 21 (4): 473–80. doi:10.1016/j.sbi.2011.05.003. PMID 21684150.
- Badran A.H. & Liu D.R. 2014. "In vivo continuous directed evolution". Current Opinion in Chemical Biology. 24C: 1–10. doi:10.1016/j.cbpa.2014.09.040. PMID 25461718.
- Leemhuis H. et al 2005. "New genotype-phenotype linkages for directed evolution of functional proteins". Current Opinion in Structural Biology. 15 (4): 472–8. doi:10.1016/j.sbi.2005.07.006. PMID 16043338.
- Lipovsek D. & Plückthun A. 2004. "In-vitro protein evolution by ribosome display and mRNA display". Journal of immunological methods. 290 (1–2): 51–67. doi:10.1016/j.jim.2004.04.008. PMID 15261571.
- Nguyen A.W. & Daugherty P.S. 2005. "Evolutionary optimization of fluorescent proteins for intracellular FRET". Nature Biotechnology. 23 (3): 355–60. doi:10.1038/nbt1066. PMID 15696158.
- Schaerli Y. & Hollfelder F. 2009. "The potential of microfluidic water-in-oil droplets in experimental biology". Molecular bioSystems. 5 (12): 1392–404. doi:10.1039/b907578j. PMID 20023716.
- Webb, Jonathan 2016. Evolutionary engineer Frances Arnold wins €1m tech prize. BBC News Science & Environment.