Tobacco mosaic virus

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Tobacco mosaic virus
TobaccoMosaicVirus.jpg
Electron micrograph of TMV particles stained to enhance visibility at 160,000x magnification
Virus classification
Group: Group IV ((+)ssRNA)
Genus: Tobamovirus

The tobacco mosaic virus (TMV) was the first virus to be discovered. That is to say, it was the first to be known as a virus.

It is a single stranded RNA virus which infects many plants, especially tobacco and other members of the family Solanaceae. The infection causes "mosaic"-like discolouration on the leaves. Although it was known from the late 19th century that an infectious disease was damaging tobacco crops, it was not until 1930 that the infectious agent was known to be a virus.

History[change | change source]

In 1886, Adolf Mayer first described the tobacco mosaic disease that could be transferred between plants, similar to bacterial infections.[1][2]

In 1892, Dmitri Ivanovsky gave the first concrete evidence for the existence of a non-bacterial infectious agent. He that infected sap remained infectious even after filtering through finest filters.[2][3]

In 1898, Martinus Beijerinck independently replicated Ivanovsky's filtration experiments and then showed that the infectious agent was able to reproduce and multiply in the host cells of the tobacco plant.[2][4] Beijerinck coined the term of "virus" to indicate that the causal agent of tobacco mosaic disease was of non-bacterial nature.

Tobacco mosaic virus was the first virus to be crystallized. It was achieved by Wendell Meredith Stanley in 1935 who also showed that TMV remains active even after crystallization.[2] For his work, he was awarded 1/3 of the Nobel Prize in Chemistry in 1946,[5] The first electron microscopical images of TMV were made in 1939.[6]

In 1955, Heinz Fraenkel-Conrat and Robley Williams showed that purified TMV RNA and its capsid (coat) protein assemble by themselves to functional viruses, indicating that this is the most stable structure (the one with the lowest free energy). The crystallographer Rosalind Franklin worked for Stanley for about a month at Berkeley, and later designed and built a model of TMV for the 1958 World's Fair at Brussels. In 1958, she speculated that the virus was hollow, not solid, and hypothesized that the RNA of TMV is single-stranded.[7] This conjecture was proved correct after her death.[8]

The investigations of tobacco mosaic disease and subsequent discovery of its viral nature were instrumental in the establishment of the general concepts of virology.[2]

References[change | change source]

  1. Mayer, Adolf (1886). "Über die Mosaikkrankheit des Tabaks." (in German). Die Landwirtschaftliche Versuchs-stationen 32: 451–467.  Translated into English in Johnson J. (ed) 1942. Phytopathological classics (St. Paul, Minnesota: American Phytopathological Society) #7, pp. 11–24.
  2. 2.0 2.1 2.2 2.3 2.4 Zaitlin, Milton (1998). "The discovery of the causal agent of the tobacco mosaic disease" (PDF). In Kung, S.D.; Yang, S.F. Discoveries in plant biology. Hong Kong: World Publishing. pp. 105–110. ISBN 978-981-02-1313-8. 
  3. Iwanowski, D. (1892). "Über die Mosaikkrankheit der Tabakspflanze" (in German, Russian). Bulletin Scientifique publié par l'Académie Impériale des Sciences de Saint-Pétersbourg / Nouvelle Serie III (St. Petersburg) 35: 67–70.  Translated into English in Johnson J. (ed) 1942. Phytopathological classics (St. Paul, Minnesota: American Phytopathological Society) #7, pp. 27–30.
  4. Beijerinck, M.W. (1898). "Über ein Contagium vivum fluidum als Ursache der Fleckenkrankheit der Tabaksblätter" (in German). Verhandelingen der Koninklyke akademie van Wettenschappen te Amsterdam 65: 1–22.  Translated into English in Johnson J. (ed) 1942. Phytopathological classics. (St. Paul, Minnesota: American Phytopathological Society) #7, pp. 33–52 (St. Paul, Minnesota)
  5. Wendell M. Stanley – Biography
  6. Kausche GA, Pfankuch E, Ruska H (May 1939). "Die Sichtbarmachung von pflanzlichem Virus im Übermikroskop". Naturwissenschaften 27 (18): 292–9. doi:10.1007/BF01493353. http://www.springerlink.com/content/v342966m49314j0h/?p=1a9e755b34b34fe0971d21e141a0e820&pi=2. 
  7. Maddox, Brenda (2002). Rosalind Franklin, the dark lady of DNA. Harper Collins. ISBN 0-06-018407-8. 
  8. http://www.agls.uidaho.edu/ebi/vdie/descr803.htm#Properties